Article
Deletion of morpholino binding sites (DeMOBS) to assess specificity of morphant phenotypes.
Scientific reports - 21 Sept 2020
Cunningham Carlee MacPherson, Bellipanni Gianfranco, Habas Raymond, Balciunas Darius
Abstract excerpt
Two complimentary approaches are widely used to study gene function in zebrafish: induction of genetic mutations, usually using targeted nucleases such as CRISPR/Cas9, and suppression of gene expression, typically using Morpholino oligomers. Neither method is perfect. Morpholinos (MOs) sometimes produce off-target or toxicity-related effects that can be mistaken for true phenotypes. Conversely, genetic mutants...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.