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Article

Lessons from morpholino-based screening in zebrafish.

Briefings in functional genomics - 1 Jul 2011

Bedell Victoria M, Westcot Stephanie E, Ekker Stephen C

Abstract excerpt

Morpholino oligonucleotides (MOs) are an effective, gene-specific antisense knockdown technology used in many model systems. Here we describe the application of MOs in zebrafish (Danio rerio) for in vivo functional characterization of gene activity. We summarize our screening experience beginning with gene target selection. We then discuss screening parameter considerations and data and database management....

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Identifiers

PMID: 21746693
DOI: 10.1093/bfgp/elr021
PMCID: PMC3144740

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