Article
The mechanism of autocatalytic activation of plant-type L-asparaginases.
The Journal of biological chemistry - 9 May 2008
Michalska Karolina, Hernandez-Santoyo Alejandra, Jaskolski Mariusz
Abstract excerpt
Plant l-asparaginases and their bacterial homologs, such as EcAIII found in Escherichia coli, form a subgroup of the N-terminal nucleophile (Ntn)-hydrolase family. In common with all Ntn-hydrolases, they are expressed as inactive precursors that undergo activation in an autocatalytic manner. The maturation process involves intramolecular hydrolysis of a single peptide bond, leading to the formation of two...
Topics
- Asparaginase
- Binding Sites
- Catalysis
- Crystallography, X-Ray
- Enzyme Activation
- Escherichia coli
- Gene Expression
- Ions
- Models, Molecular
- Mutation
- Plants
- Protein Structure, Quaternary
- Protein Structure, Tertiary