Article
Evolutionary action of mutations reveals antimicrobial resistance genes in Escherichia coli.
Nature communications - 9 Jun 2022
Marciano David C, Wang Chen, Hsu Teng-Kuei, Bourquard Thomas, Atri Benu, Nehring Ralf B, Abel Nicholas S, Bowling Elizabeth A, Chen Taylor J, Lurie Pamela D, Katsonis Panagiotis, Rosenberg Susan M, Herman Christophe, Lichtarge Olivier
Abstract excerpt
Since antibiotic development lags, we search for potential drug targets through directed evolution experiments. A challenge is that many resistance genes hide in a noisy mutational background as mutator clones emerge in the adaptive population. Here, to overcome this noise, we quantify the impact of mutations through evolutionary action (EA). After sequencing ciprofloxacin or colistin resistance strains grown...
Topics
- Anti-Bacterial Agents
- Ciprofloxacin
- Colistin
- Drug Resistance, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Microbial Sensitivity Tests
- Mutation