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Article

Non-lethal PCR genotyping of single Drosophila.

BioTechniques - 1 Apr 2009

Carvalho Gil B, Ja William W, Benzer Seymour

Abstract excerpt

In Drosophila, genetic techniques relying on stochastic chromosomal rearrangements involve the generation and screening of a large number of fly stocks to isolate a few lines of interest. Here, we describe a PCR-based method allowing non-lethal molecular characterization of single flies. Using this procedure, individual candidate recombinant animals can be genotyped and selected one generation earlier than with...

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Identifiers

PMID: 19450239
DOI: 10.2144/000113088
PMCID: PMC2793178

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