Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Preanalytical blood sample workup for cell-free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics.

Cancer medicine - 1 Oct 2017

van Ginkel Joost H, van den Broek Daan A, van Kuik Joyce, Linders Dorothé, de Weger Roel, Willems Stefan M, Huibers Manon M H

Abstract excerpt

In current molecular cancer diagnostics, using blood samples of cancer patients for the detection of genetic alterations in plasma (cell-free) circulating tumor DNA (ctDNA) is an emerging practice. Since ctDNA levels in blood are low, highly sensitive Droplet Digital PCR (ddPCR) can be used for detecting rare mutational targets. In order to perform ddPCR on blood samples, a standardized procedure for processing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biomarkers, Tumor
Share to Case-Control Studies

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Circulating Tumor DNA

Share to Liquid Biopsy

Share to Lung Neoplasms

Share to Molecular Diagnostic Techniques

Share to Neoplasms

Share to Workflow

Source

Open in PubMed Open full text

Identifiers

PMID: 28940814
DOI: 10.1002/cam4.1184
PMCID: PMC5633557

Genes

BRAF
EGFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Preanalytical blood sample workup for cell-free DNA analysis using Droplet Digital PCR for future molecular cancer diagnostics.PMID 28940814Read ↗

Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective.Semantic proximity Read ↗

Evaluation of pre-analytical factors affecting plasma DNA analysisSemantic proximity Read ↗

Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsies.Semantic proximity Read ↗

Current and Emerging Applications of Droplet Digital PCR in Oncology: An Updated Review.Semantic proximity Read ↗

A Study of Pre-Analytical Variables and Optimization of Extraction Method for Circulating Tumor DNA Measurements by Digital Droplet PCRSemantic proximity Read ↗

Current and Emerging Applications of Droplet Digital PCR in Oncology.Semantic proximity Read ↗

Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction.Semantic proximity Read ↗

Optimized (Pre) Analytical Conditions and Workflow for Droplet Digital PCR Analysis of Cell-Free DNA from Patients with Suspected Lung CarcinomaSemantic proximity Read ↗

Comparative study of droplet-digital PCR and absolute Q digital PCR for ctDNA detection in early-stage breast cancer patients.Semantic proximity Read ↗

Total DNA input is a crucial determinant of the sensitivity of plasma cell-free DNA EGFR mutation detection using droplet digital PCRSemantic proximity Read ↗

A field guide for cancer diagnostics using cell-free DNA: From principles to practice and clinical applications.Semantic proximity Read ↗

Clinical validation and implementation of droplet digital PCR for the detection of BRAF mutations from cell-free DNA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.