Article
Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function.
Science (New York, N.Y.) - 13 May 2022
Eraslan Gökcen, Drokhlyansky Eugene, Anand Shankara, Fiskin Evgenij, Subramanian Ayshwarya, Slyper Michal, Wang Jiali, Van Wittenberghe Nicholas, Rouhana John M, Waldman Julia, Ashenberg Orr, Lek Monkol, Dionne Danielle, Win Thet Su, Cuoco Michael S, Kuksenko Olena, Tsankov Alexander M, Branton Philip A, Marshall Jamie L, Greka Anna, Getz Gad, Segrè Ayellet V, Aguet François, Rozenblatt-Rosen Orit, Ardlie Kristin G, Regev Aviv
Abstract excerpt
Understanding gene function and regulation in homeostasis and disease requires knowledge of the cellular and tissue contexts in which genes are expressed. Here, we applied four single-nucleus RNA sequencing methods to eight diverse, archived, frozen tissue types from 16 donors and 25 samples, generating a cross-tissue atlas of 209,126 nuclei profiles, which we integrated across tissues, donors, and laboratory...
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