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Article

Residues located in the primase domain of the bacteriophage T7 primase-helicase are essential for loading the hexameric complex onto DNA.

The Journal of biological chemistry - 1 Jun 2022

Hernandez Alfredo J, Lee Seung-Joo, Thompson Noah J, Griffith Jack D, Richardson Charles C

Abstract excerpt

The T7 primase-helicase plays a pivotal role in the replication of T7 DNA. Using affinity isolation of peptide-nucleic acid crosslinks and mass spectrometry, we identify protein regions in the primase-helicase and T7 DNA polymerase that form contacts with the RNA primer and DNA template. The contacts between nucleic acids and the primase domain of the primase-helicase are centered in the RNA polymerase subdomain...

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Identifiers

PMID: 35500649
DOI: 10.1016/j.jbc.2022.101996
PMCID: PMC9198812

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