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Article

Structure of an open conformation of T7 DNA polymerase reveals novel structural features regulating primer-template stabilization at the polymerization active site.

The Biochemical journal - 16 Jul 2021

Juarez-Quintero Víctor, Peralta-Castro Antolín, Benítez Cardoza Claudia G, Ellenberger Tom, Brieba Luis G

Abstract excerpt

The crystal structure of full-length T7 DNA polymerase in complex with its processivity factor thioredoxin and double-stranded DNA in the polymerization active site exhibits two novel structural motifs in family-A DNA polymerases: an extended β-hairpin at the fingers subdomain, that interacts with the DNA template strand downstream the primer-terminus, and a helix-loop-helix motif (insertion1) located between...

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Source

Identifiers

PMID: 34160020
DOI: 10.1042/BCJ20200922

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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