Article
The Structure-Function Relationship of Human Bleomycin Hydrolase: Mutation of a Cysteine Protease into a Serine Protease.
Chembiochem : a European journal of chemical biology - 20 Jun 2022
Zheng Yi-Zhen, Cui Jingxuan, Wang Yung-Lin, Huang Szu-Jo, Lin En-Chi, Huang Sheng-Cih, Rudolf Jeffrey D, Yan Xiaohui, Chang Chin-Yuan
Abstract excerpt
Human bleomycin hydrolase (hBH) catalyzes deamidation of the anticancer drug bleomycins (BLM). This enzyme is involved in BLM detoxification and drug resistance. Herein, we report the putative BLM-binding site and catalytic mechanism of hBH. The crystal structures and biochemical studies suggest that hBH cleaves its C-terminal residue without significant preference for the type of amino acid, and therefore can...
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