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Article

A real-time PCR method to genotype mutant mouse models with altered affinity for cardiotonic steroids on the Na,K-ATPase.

PloS one - 1 Jan 2022

Chomczynski Peter W, Vires Kianna M, Rymaszewski Michal, Heiny Judith A

Abstract excerpt

The highly conserved, cardiotonic steroid binding site (also termed ouabain binding site) on the primary α subunit of Na,K-ATPase plays a receptor signaling role in a range of vital cell processes and is a therapeutic target for human disease. Mouse lines with altered affinity for cardiotonic steroids on the α1 or α2 subunit isoform of Na,K-ATPase, without any change in pump activity, were developed by the late...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 35446892
DOI: 10.1371/journal.pone.0267348
PMCID: PMC9022855

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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