Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique.

doi:10.1111/dgd.12778

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Article

Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique.

Development, growth & differentiation - 1 May 2022

Tanouchi Mikio, Igawa Takeshi, Suzuki Nanoka, Suzuki Makoto, Hossain Nusrat, Ochi Haruki, Ogino Hajime

Abstract excerpt

The CRISPR/Cas9 method has become popular for gene disruption experiments in Xenopus laevis. However, the experimental conditions that influence the efficiency of CRISPR/Cas9 remain unclear. To that end, we developed an image analysis technique for the semi-quantitative evaluation of the pigment...

Topics

Animals
CRISPR-Cas Systems
Gene Editing
Phenotype
RNA, Messenger
Xenopus laevis

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