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Article

Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique.

Development, growth & differentiation - 1 May 2022

Tanouchi Mikio, Igawa Takeshi, Suzuki Nanoka, Suzuki Makoto, Hossain Nusrat, Ochi Haruki, Ogino Hajime

Abstract excerpt

The CRISPR/Cas9 method has become popular for gene disruption experiments in Xenopus laevis. However, the experimental conditions that influence the efficiency of CRISPR/Cas9 remain unclear. To that end, we developed an image analysis technique for the semi-quantitative evaluation of the pigment phenotype resulting from the disruption of tyrosinase genes in X. laevis using a CRISPR/Cas9 approach, and then...

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Identifiers

PMID: 35338712
DOI: 10.1111/dgd.12778
PMCID: PMC11520957

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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