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Article

Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein.

Microbiology spectrum - 22 Dec 2021

Davis James J, Long S Wesley, Christensen Paul A, Olsen Randall J, Olson Robert, Shukla Maulik, Subedi Sishir, Stevens Rick, Musser James M

Abstract excerpt

The ARTIC Network provides a common resource of PCR primer sequences and recommendations for amplifying SARS-CoV-2 genomes. The initial tiling strategy was developed with the reference genome Wuhan-01, and subsequent iterations have addressed areas of low amplification and sequence drop out. Recently, a new version (V4) was released, based on new variant genome sequences, in response to the realization that some...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34878296
DOI: 10.1128/Spectrum.01803-21
PMCID: PMC8653831

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