Article

Analysis of the ARTIC version 3 and version 4 SARS-CoV-2 primers and their impact on the detection of the G142D amino acid substitution in the spike protein

2021-09-28

Abstract excerpt

<h4>ABSTRACT</h4> The ARTIC Network provides a common resource of PCR primer sequences and recommendations for amplifying SARS-CoV-2 genomes. The initial tiling strategy was developed with the reference genome Wuhan-01, and subsequent iterations have addressed areas of low amplification and sequence drop out. Recently, a new version (V4) was released, based on new variant genome sequences, in response to the real...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bc66e7e8-cf73-55f8-b1a2-e96f130b6ecb
DOI: 10.1101/2021.09.27.461949

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Analysis of the ARTIC version 3 and version 4 SARS-CoV-2 primers and their impact on the detection of the G142D amino acid substitution in the spike proteinDOI 10.1101/2021.09.27.461949Read

Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein.Semantic proximity Read

High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant Determination.Semantic proximity Read

Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage-defining mutations.Semantic proximity Read

HiSpike: A high-throughput cost effective sequencing method for the SARS-CoV-2 spike geneSemantic proximity Read

Discriminatory Weight of SNPs in Spike SARS-CoV-2 Variants: A Technically Rapid, Unambiguous, and Bioinformatically Validated Laboratory Approach.Semantic proximity Read

Targeted Amplification and Genetic Sequencing of the Severe Acute Respiratory Syndrome Coronavirus 2 Surface GlycoproteinSemantic proximity Read

SARS-CoV-2 variants of concern and spike protein mutational dynamics in a Swedish cohort during 2021, studied by Nanopore sequencing.Semantic proximity Read

HiSpike: A high-throughput cost effective sequencing method for the SARS-CoV-2 spike geneSemantic proximity Read

Analysis of the ARTIC V4 and V4.1 SARS-CoV-2 primers and their impact on the detection of Omicron BA.1 and BA.2 lineage defining mutationsSemantic proximity Read

Real-time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with Variants of ConcernSemantic proximity Read

Evaluation of an optimized protocol and Illumina ARTIC V4 primer pool for sequencing of SARS-CoV-2 using COVIDSeq™ and DRAGEN™ COVID Lineage App workflowSemantic proximity Read

Development of an efficient Sanger sequencing-based assay for detecting SARS-CoV-2 spike mutations.Semantic proximity Read