Article
GBS-MeDIP: A protocol for parallel identification of genetic and epigenetic variation in the same reduced fraction of genomes across individuals.
STAR protocols - 18 Mar 2022
Rezaei Shiva, Uffenorde Julia, Gimm Oliver, Hosseinpour Feizi Mohammad Ali, Miemczyk Stefan, Coutinho Luiz Lehmann, Jensen Per, Guerrero-Bosagna Carlos, Pértille Fábio
Abstract excerpt
The GBS-MeDIP protocol combines two previously described techniques, Genotype-by-Sequencing (GBS) and Methylated-DNA-Immunoprecipitation (MeDIP). Our method allows for parallel and cost-efficient interrogation of genetic and methylomic variants in the DNA of many reduced genomes, taking advantage of the barcoding of DNA samples performed in the GBS and the subsequent creation of DNA pools, then used as an input...
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