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CRISPR-Mediated Enzyme Fragment Complementation Assay for Quantification of the Stability of Splice Isoforms.

Chembiochem : a European journal of chemical biology - 4 May 2022

Tang Zhichao, Hegde Shalakha, Zhao Junxing, Zhu Shoutian, Johnson Kristen A, Lorson Christian L, Wang Jingxin

Abstract excerpt

Small-molecule splicing modulators exemplified by an FDA-approved drug, risdiplam, are a new pharmacological modality for regulating the expression and stability of splice isoforms. We report a CRISPR-mediated enzyme fragment complementation (EFC) assay to quantify the splice isoform stability. The EFC assay harnessed a 42 amino acid split of a β-galactosidase (designate α-tag), which could be fused at the...

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PMID: 35235240
DOI: 10.1002/cbic.202200012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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