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Evaluation of LipL32 and LigA/LigB Knockdown Mutants in Leptospira interrogans Serovar Copenhageni: Impacts to Proteome and Virulence

2 Feb 2022

Abstract excerpt

Leptospirosis is a worldwide zoonosis caused by pathogenic species of the genus Leptospira . The recent application of CRISPR interference (CRISPRi) to Leptospira facilitates targeted gene silencing and provides a new tool to investigate pathogenic mechanisms of leptospirosis. CRISPRi relies on the expression of a catalytically “dead” Cas9 (dCas9) and a single-guide RNA (sgRNA). Previously, our group generated a...

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PMID: 35185824
DOI: 10.3389/fmicb.2021.799012

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