Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32

Abstract excerpt

BACKGROUND: One of the key barriers preventing rapid diagnosis of leptospirosis is the lack of available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined with isothermal amplification to detect leptospires from extracted patient DNA samples....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Leprosy Research and Treatment
• Share to Leptospirosis research and findings
• Share to Syphilis Diagnosis and Treatment