Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation.

Scientific reports - 5 Feb 2025

Fernandes L G V, Nascimento A L T O, Nally J E

Abstract excerpt

Expanding the genetic toolkit for Leptospira spp. is a crucial step toward advancing our understanding of the biology and virulence of these atypical bacteria. Pathogenic Leptospira are responsible for over 1 million human leptospirosis cases annually and significantly impact domestic animals. Bovine leptospirosis causes substantial financial losses due to abortion, stillbirths, and suboptimal reproductive...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to CRISPR-Cas Systems
Share to Gene Editing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Knockout Techniques

Share to Leptospira

Share to Leptospirosis

Share to Mutation

Source

Open in PubMed Open full text

Identifiers

PMID: 39910196
DOI: 10.1038/s41598-025-88633-w
PMCID: PMC11799391

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Induced protein expression in Leptospira spp. and its application to CRISPR/Cas9 mutant generation.PMID 39910196Read ↗

CRISPR-prime editing, a versatile genetic tool to create specific mutations with a single nucleotide resolution in Leptospira.Semantic proximity Read ↗

Genetic manipulation of pathogenic Leptospira: CRISPR interference (CRISPRi)-mediated gene silencing and rapid mutant recovery at 37 °C.Semantic proximity Read ↗

Gene silencing based on RNA-guided catalytically inactive Cas9 (dCas9): a new tool for genetic engineering in LeptospiraSemantic proximity Read ↗

Evaluation of LipL32 and LigA/LigB Knockdown Mutants in Leptospira interrogans Serovar Copenhageni: Impacts to Proteome and VirulenceSemantic proximity Read ↗

Bioinformatic Identification of CRISPR-Cas Systems in <em>Leptospira</em>: An Update on Their Distribution Across 77 SpeciesSemantic proximity Read ↗

Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9Semantic proximity Read ↗

Whole genome sequencing and de novo assembly of three virulent Indian isolates of Leptospira.Semantic proximity Read ↗

Dual nuclease activity of a Cas2 protein in CRISPR –Cas subtype I‐B of Leptospira interrogansSemantic proximity Read ↗

An efficient in vivo -inducible CRISPR interference system for group A Streptococcus genetic analysis and pathogenesis studiesSemantic proximity Read ↗

A genome-wide CRISPR screen identified host genes essential for intracellular Brucella survivalSemantic proximity Read ↗

Impact of CRISPR-Cas9-Based Genome Engineering in Farm AnimalsSemantic proximity Read ↗

CRISPR/Cas9 knockout and editing of the major cysteine protease in <i>Entamoeba histolytica</i>Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.