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Article

Towards standardization of next-generation sequencing of FFPE samples for clinical oncology: intrinsic obstacles and possible solutions.

Journal of translational medicine - 31 Jan 2017

Ivanov Maxim, Laktionov Konstantin, Breder Valery, Chernenko Polina, Novikova Ekaterina, Telysheva Ekaterina, Musienko Sergey, Baranova Ancha, Mileyko Vladislav

Abstract excerpt

BACKGROUND: Next generation sequencing has a potential to revolutionize the management of cancer patients within the framework of precision oncology. Nevertheless, lack of standardization decelerated entering of the technology into the clinical testing space. Here we dissected a number of common problems of NGS diagnostics in oncology and introduced ways they can be resolved. METHODS: DNA was extracted from 26...

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PMID: 28137276
DOI: 10.1186/s12967-017-1125-8
PMCID: PMC5282851

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EGFR

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