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Article

Development of an Outward Proton Pumping Rhodopsin with a New Record in Thermostability by Means of Amino Acid Mutations.

The journal of physical chemistry. B - 10 Feb 2022

Yasuda Satoshi, Akiyama Tomoki, Kojima Keiichi, Ueta Tetsuya, Hayashi Tomohiko, Ogasawara Satoshi, Nagatoishi Satoru, Tsumoto Kouhei, Kunishima Naoki, Sudo Yuki, Kinoshita Masahiro, Murata Takeshi

Abstract excerpt

We have developed a methodology for identifying further thermostabilizing mutations for an intrinsically thermostable membrane protein. The methodology comprises the following steps: (1) identifying thermostabilizing single mutations (TSSMs) for residues in the transmembrane region using our physics-based method; (2) identifying TSSMs for residues in the extracellular and intracellular regions, which are in...

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PMID: 35089040
DOI: 10.1021/acs.jpcb.1c08684

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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