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Article

An ultra-stable cytoplasmic antibody engineered for in vivo applications

17 Jan 2020

Abstract excerpt

Targeting cytoplasmic protein-protein interactions with antibodies remains technically challenging, since antibodies expressed in the cytosol frequently form insoluble aggregates. Existing engineering methods are based on the notion that the estimated net charge at pH 7.4 affects stability; as such, they are unable to overcome this problem. Herein, we report a versatile method for engineering an ultra-stable...

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PMID: 31953402
DOI: 10.1038/s41467-019-13654-9

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