Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning.

ACS synthetic biology - 21 Jan 2022

Ogawa Yuki, Katsuyama Yohei, Ohnishi Yasuo

Abstract excerpt

Deep mutational scanning is a method for protein engineering. Here, we applied it to alter the ligand specificity of the transcriptional regulator XylS from Pseudomonas putida to recognize p-toluic acid instead of the native ligand m-toluic acid. For this purpose, we used an antibiotic resistance gene-based dual screening system, which was constructed for the directed evolution of XylS toward the above-mentioned...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacterial Proteins
Share to Ligands
Share to Mutation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Plasmids

Share to Protein Engineering

Share to Pseudomonas putida

Source

Identifiers

PMID: 34964613
DOI: 10.1021/acssynbio.1c00564

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning.PMID 34964613Read ↗

Switching the Ligand Specificity of the Biosensor XylS from meta to para-Toluic Acid through Directed Evolution Exploiting a Dual Selection System.Semantic proximity Read ↗

Identification of critical amino-terminal regions of XylS. The positive regulator encoded by the TOL plasmid.Semantic proximity Read ↗

Identifying LasR Quorum Sensors with Improved Signal Specificity by Mapping the Sequence-Function Landscape.Semantic proximity Read ↗

A la carte transcriptional regulators: unlocking responses of the prokaryotic enhancer-binding protein XylR to non-natural effectors.Semantic proximity Read ↗

Reverse engineering of an aspirin-responsive regulator in bacteriaSemantic proximity Read ↗

Engineering an allosteric transcription factor to respond to new ligands.Semantic proximity Read ↗

Directed evolution of a synthetic phylogeny of programmable Trp repressors.Semantic proximity Read ↗

Generation of orthogonally selective bacterial riboswitches by targeted mutagenesis and in vivo screening.Semantic proximity Read ↗

Mapping the phenotypic landscape of a transcriptional repressor using deep mutational scanning and growth-based quantitative sequencing.Semantic proximity Read ↗

Speeding up directed evolution: Combining the advantages of solid-phase combinatorial gene synthesis with statistically guided reduction of screening effort.Semantic proximity Read ↗

High-throughput screening system based on phenolics-responsive transcription activator for directed evolution of organophosphate-degrading enzymes.Semantic proximity Read ↗

Rational Protein Engineering Guided by Deep Mutational Scanning.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.