Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning.

doi:10.1021/acssynbio.1c00564

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Article

Engineering of the Ligand Specificity of Transcriptional Regulator XylS by Deep Mutational Scanning.

ACS synthetic biology - 21 Jan 2022

Ogawa Yuki, Katsuyama Yohei, Ohnishi Yasuo

Abstract excerpt

Deep mutational scanning is a method for protein engineering. Here, we applied it to alter the ligand specificity of the transcriptional regulator XylS from Pseudomonas putida to recognize p-toluic acid instead of the native ligand m-toluic acid. For this purpose, we used an antibiotic resistance gene-based dual screening system, which was constructed for the directed evolution of XylS toward the above-mentioned...

Topics

Bacterial Proteins
Ligands
Mutation
Plasmids
Protein Engineering
Pseudomonas putida

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Open in PubMed

Identifiers

PMID: 34964613
DOI: 10.1021/acssynbio.1c00564

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