Article
Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System.
Journal of visualized experiments : JoVE - 10 Dec 2021
Pace James B, Huang Nian N, Séguin Jacqueline P, Esquina Candi, Olin Erin, Zhu Guangyu, Carr Grant
Abstract excerpt
Full-length huntingtin (FL HTT) is a large (aa 1-3,144), ubiquitously expressed, polyglutamine (polyQ)-containing protein with a mass of approximately 350 kDa. While the cellular function of FL HTT is not entirely understood, a mutant expansion of the polyQ tract above ~36 repeats is associated with Huntington's disease (HD), with the polyQ length correlating roughly with the age of onset. To better understand...
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