Article
The BRCT domain of PARP1 binds intact DNA and mediates intrastrand transfer.
Molecular cell - 16 Dec 2021
Rudolph Johannes, Muthurajan Uma M, Palacio Megan, Mahadevan Jyothi, Roberts Genevieve, Erbse Annette H, Dyer Pamela N, Luger Karolin
Abstract excerpt
PARP1 is a key player in the response to DNA damage and is the target of clinical inhibitors for the treatment of cancers. Binding of PARP1 to damaged DNA leads to activation wherein PARP1 uses NAD+ to add chains of poly(ADP-ribose) onto itself and other nuclear proteins. PARP1 also binds abundantly to intact DNA and chromatin, where it remains enzymatically inactive. We show that intact DNA makes contacts with...
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