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Article

MicroSEC filters sequence errors for formalin-fixed and paraffin-embedded samples.

Communications biology - 15 Dec 2021

Ikegami Masachika, Kohsaka Shinji, Hirose Takeshi, Ueno Toshihide, Inoue Satoshi, Kanomata Naoki, Yamauchi Hideko, Mori Taisuke, Sekine Shigeki, Inamoto Yoshihiro, Yatabe Yasushi, Kobayashi Hiroshi, Tanaka Sakae, Mano Hiroyuki

Abstract excerpt

The clinical sequencing of tumors is usually performed on formalin-fixed, paraffin-embedded samples and results in many sequencing errors. We identified that most of these errors are detected in chimeric reads caused by single-strand DNA molecules with microhomology. During the end-repair step of library preparation, mutations are introduced by the mis-annealing of two single-strand DNA molecules comprising...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34912045
DOI: 10.1038/s42003-021-02930-4
PMCID: PMC8674242

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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