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More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replication.

Scientific reports - 13 Dec 2021

Luk Ka-Cheung, Gersch Jeffrey, Harris Barbara J, Holzmayer Vera, Mbanya Dora, Sauleda Silvia, Rodgers Mary A, Cloherty Gavin

Abstract excerpt

HBV produces unspliced and spliced RNAs during replication. Encapsidated spliced RNA is converted into DNA generating defective virions that are detected in plasma and associated with HCC development. Herein we describe a quantitative real-time PCR detection of splice variant SP1 DNA/RNA in HBV plasma. Three PCR primers/probe sets were designed detecting the SP1 variants, unspliced core, or X gene. Plasmids...

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Source

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Identifiers

PMID: 34903774
DOI: 10.1038/s41598-021-03304-w
PMCID: PMC8668879

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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