Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High-dimensional droplet digital PCR of multiple hepatitis B splice variants in serum, liver, and tissue culture.

Journal of virological methods - 1 Jul 2025

Grudda Tanner, Jiang Chenkai, Lo Che-Min, Skinner Nicole E, Sulkowski Mark S, Balagopal Ashwin, Thio Chloe L

Abstract excerpt

Hepatitis B virus (HBV) affects approximately 2 billion individuals; 254 million have chronic infection. The roles of spliced HBV (spHBV) RNAs in human infection are poorly understood partly due to limitations in quantitative methods. We designed and multiplexed long amplicon (0.3-2kbp) ddPCR assays that simultaneously quantify the most frequently observed spHBV isoforms (sp1, sp2, sp3, sp8, sp9) alongside...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genotype
Share to Hepatitis B

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hepatitis B virus

Share to Real-Time Polymerase Chain Reaction

Share to RNA Splicing

Share to RNA, Viral

Source

Open in PubMed Open full text

Identifiers

PMID: 40209859
DOI: 10.1016/j.jviromet.2025.115155
PMCID: PMC12455858

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

High-dimensional droplet digital PCR of multiple hepatitis B splice variants in serum, liver, and tissue culture.PMID 40209859Read ↗

Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypesSemantic proximity Read ↗

Hepatitis B virus serum RNA transcript isoform composition and proportion in chronic hepatitis B patients by nanopore long-read sequencingSemantic proximity Read ↗

Integrated DNA estimation in tissue biopsy and detection in liquid biopsy by HBV-targeted NGS assaySemantic proximity Read ↗

Quantitative analysis of the splice variants expressed by the major hepatitis B virus genotypes.Semantic proximity Read ↗

Hepatitis B Virus RNA Profiles in Liver Biopsies by Digital Polymerase Chain ReactionSemantic proximity Read ↗

Single-cell RNA sequencing of liver fine-needle aspirates captures immune diversity in the blood and liver in chronic hepatitis B patientsSemantic proximity Read ↗

Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and MisunderstoodSemantic proximity Read ↗

Method for Quantitative HDV RNA Detection: I, Manual Workflow (Serum and Liver Tissue) and II, Fully Automated High Throughput Workflow for Diagnostic Use.Semantic proximity Read ↗

HDV RNA assays: Performance characteristics, clinical utility, and challengesSemantic proximity Read ↗

More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replication.Semantic proximity Read ↗

Improved hepatitis delta virus genome characterization by single molecule full-length genome sequencing combined with VIRiONT pipeline.Semantic proximity Read ↗

Comparison of droplet digital PCR to real-time PCR for quantification of hepatitis B virus DNASemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.