Article
The human T24 Ha-ras1 oncogene: a study of the effects of overexpression of the mutated ras gene product in rodent cells.
Anticancer research - 1 Jan 2000
Spandidos D A
Abstract excerpt
The human T24 Ha-ras1 oncogene was inserted into a high expression vector which carried the aminoglycoside phosphotransferase (aph) gene as a selectable market. This recombinant was introduced into early passage rodent cells by the calcium-phosphate technique. Under the selection pressure of geneticin foci appeared of morphologically altered cells which became immortalized, anchorage independent and tumourigenic....
Topics
- Animals
- Cell Transformation, Neoplastic
- Cells, Cultured
- Cricetinae
- Female
- Humans
- Mutation
- Neoplasm Proteins
- Oncogene Protein p21(ras)
- Oncogenes
- Phenotype
- Rats