Article
The human T24 Ha-ras1 oncogene: a study of the effects of overexpression of the mutated ras gene product in rodent cells.
Anticancer research - 1 Jan 2000
Spandidos D A
Abstract excerpt
The human T24 Ha-ras1 oncogene was inserted into a high expression vector which carried the aminoglycoside phosphotransferase (aph) gene as a selectable market. This recombinant was introduced into early passage rodent cells by the calcium-phosphate technique. Under the selection pressure of geneticin foci appeared of morphologically altered cells which became immortalized, anchorage independent and tumourigenic....
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