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Article

Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.

Proceedings of the National Academy of Sciences of the United States of America - 7 Dec 2021

Wan Lei, Chen Xi, Almers Wolfhard

Abstract excerpt

Syntaxin helps in catalyzing membrane fusion during exocytosis. It also forms clusters in the plasma membrane, where both its transmembrane and SNARE domains are thought to homo-oligomerize. To study syntaxin clustering in live PC12 cells, we labeled granules with neuropeptide-Y-mCherry and syntaxin clusters with syntaxin-1a green fluorescent protein (GFP). Abundant clusters appeared under total internal...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34857632
DOI: 10.1073/pnas.2025748118
PMCID: PMC8670517

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Munc18-dependent and -independent clustering of syntaxin in the plasma membrane of cultured endocrine cells.PMID 34857632Read ↗

Syntaxins on granules promote docking of granules via interactions with munc18.Semantic proximity Read ↗

Control of fusion pore dynamics during exocytosis by Munc18.Semantic proximity Read ↗

Fluorescence resonance energy transfer reports properties of syntaxin1a interaction with Munc18-1 in vivo.Semantic proximity Read ↗

Rescue of Munc18-1 and -2 double knockdown reveals the essential functions of interaction between Munc18 and closed syntaxin in PC12 cells.Semantic proximity Read ↗

Munc18-1 regulates early and late stages of exocytosis via syntaxin-independent protein interactions.Semantic proximity Read ↗

A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3.Semantic proximity Read ↗

Role of H(abc) domain in membrane trafficking and targeting of syntaxin 1A.Semantic proximity Read ↗

Munc18-1 induces conformational changes of syntaxin-1 in multiple intermediates for SNARE assembly.Semantic proximity Read ↗

Syntaxin/Munc18 interactions in the late events during vesicle fusion and release in exocytosis.Semantic proximity Read ↗

Role of Munc13-4 as a Ca2+-dependent tether during platelet secretion.Semantic proximity Read ↗

Extension of Helix 12 in Munc18-1 Induces Vesicle Priming.Semantic proximity Read ↗

SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion.Semantic proximity Read ↗

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