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Article

Munc18-1 induces conformational changes of syntaxin-1 in multiple intermediates for SNARE assembly.

Scientific reports - 15 Jul 2020

Lee Sanghwa, Shin Jonghyeok, Jung Younghun, Son Heyjin, Shin Jaeil, Jeong Cherlhyun, Kweon Dae-Hyuk, Shin Yeon-Kyun

Abstract excerpt

In neuronal exocytosis, SNARE assembly into a stable four-helix bundle drives membrane fusion. Previous studies have revealed that the SM protein Munc18-1 plays a critical role for precise SNARE assembly with the help of Munc13-1, but the underlying mechanism remains unclear. Here, we used single-molecule FRET assays with a nanodisc membrane reconstitution system to investigate the conformational dynamics of...

Topics

Animals
Exocytosis
Fluorescence Resonance Energy Transfer
Kinetics
Membrane Fusion
Munc18 Proteins
Mutation
Nanotechnology
Neurons
Protein Binding
Protein Conformation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Domains

Rats

Syntaxin 1

Source

Open in PubMed Open full text

Identifiers

PMID: 32669573
DOI: 10.1038/s41598-020-68476-3
PMCID: PMC7363831

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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Munc18-1 induces conformational changes of syntaxin-1 in multiple intermediates for SNARE assembly.PMID 32669573Read ↗

Selective activation of cognate SNAREpins by Sec1/Munc18 proteins.Semantic proximity Read ↗

SNARE bundle and syntaxin N-peptide constitute a minimal complement for Munc18-1 activation of membrane fusion.Semantic proximity Read ↗

The trans-SNARE-regulating function of Munc18-1 is essential to synaptic exocytosis.Semantic proximity Read ↗

Double-Transmembrane Domain of SNAREs Decelerates the Fusion by Increasing the Protein-Lipid Mismatch.Semantic proximity Read ↗

Syntaxin N-terminal peptide motif is an initiation factor for the assembly of the SNARE-Sec1/Munc18 membrane fusion complex.Semantic proximity Read ↗

Conformational switch of syntaxin-1 controls synaptic vesicle fusion.Semantic proximity Read ↗

The functions of Munc18-1 in regulated exocytosis.Semantic proximity Read ↗

Control of fusion pore dynamics during exocytosis by Munc18.Semantic proximity Read ↗

Dynamic conformational changes in munc18 prevent syntaxin binding.Semantic proximity Read ↗

Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1.Semantic proximity Read ↗

Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans.Semantic proximity Read ↗

Structural transitions in the synaptic SNARE complex during Ca2+-triggered exocytosis.Semantic proximity Read ↗

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