Discrimination between 34 of 36 Possible Combinations of Three C>T SNP Genotypes in the MGMT Promoter by High Resolution Melting Analysis Coupled with Pyrosequencing Using A Single Primer Set.

doi:10.3390/ijms222212527

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Article

Discrimination between 34 of 36 Possible Combinations of Three C>T SNP Genotypes in the MGMT Promoter by High Resolution Melting Analysis Coupled with Pyrosequencing Using A Single Primer Set.

International journal of molecular sciences - 20 Nov 2021

Zappe Katja, Pirker Christine, Miedl Heidi, Schreiber Martin, Heffeter Petra, Pfeiler Georg, Hacker Stefan, Haslik Werner, Spiegl-Kreinecker Sabine, Cichna-Markl Margit

Abstract excerpt

Due to its cost-efficiency, high resolution melting (HRM) analysis plays an important role in genotyping of candidate single nucleotide polymorphisms (SNPs). Studies indicate that HRM analysis is not only suitable for genotyping individual SNPs, but also allows genotyping of multiple SNPs in one and the same amplicon, although with limited discrimination power. By targeting the three C>T SNPs rs527559815,...

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PMID: 34830407
DOI: 10.3390/ijms222212527
PMCID: PMC8621402

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