Article
De novo identification of mammalian ciliary motility proteins using cryo-EM.
Cell - 11 Nov 2021
Gui Miao, Farley Hannah, Anujan Priyanka, Anderson Jacob R, Maxwell Dale W, Whitchurch Jonathan B, Botsch J Josephine, Qiu Tao, Meleppattu Shimi, Singh Sandeep K, Zhang Qi, Thompson James, Lucas Jane S, Bingle Colin D, Norris Dominic P, Roy Sudipto, Brown Alan
Abstract excerpt
Dynein-decorated doublet microtubules (DMTs) are critical components of the oscillatory molecular machine of cilia, the axoneme, and have luminal surfaces patterned periodically by microtubule inner proteins (MIPs). Here we present an atomic model of the 48-nm repeat of a mammalian DMT, derived from a cryoelectron microscopy (cryo-EM) map of the complex isolated from bovine respiratory cilia. The structure...
Topics
- Amino Acid Sequence
- Animals
- Cattle
- Cilia
- Cryoelectron Microscopy
- Dyneins
- Embryo, Mammalian
- Female
- Male
- Mammals
- Mice, Inbred C57BL