Article
Design, synthesis, and testing toward a 57-codon genome.
Science (New York, N.Y.) - 19 Aug 2016
Ostrov Nili, Landon Matthieu, Guell Marc, Kuznetsov Gleb, Teramoto Jun, Cervantes Natalie, Zhou Minerva, Singh Kerry, Napolitano Michael G, Moosburner Mark, Shrock Ellen, Pruitt Benjamin W, Conway Nicholas, Goodman Daniel B, Gardner Cameron L, Tyree Gary, Gonzales Alexandra, Wanner Barry L, Norville Julie E, Lajoie Marc J, Church George M
Abstract excerpt
Recoding--the repurposing of genetic codons--is a powerful strategy for enhancing genomes with functions not commonly found in nature. Here, we report computational design, synthesis, and progress toward assembly of a 3.97-megabase, 57-codon Escherichia coli genome in which all 62,214 instances of seven codons were replaced with synonymous alternatives across all protein-coding genes. We have validated 63% of...
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