Article
High-dimensional and large-scale phenotyping of yeast mutants.
Proceedings of the National Academy of Sciences of the United States of America - 27 Dec 2005
Ohya Yoshikazu, Sese Jun, Yukawa Masashi, Sano Fumi, Nakatani Yoichiro, Saito Taro L, Saka Ayaka, Fukuda Tomoyuki, Ishihara Satoru, Oka Satomi, Suzuki Genjiro, Watanabe Machika, Hirata Aiko, Ohtani Miwaka, Sawai Hiroshi, Fraysse Nicolas, Latgé Jean-Paul, François Jean M, Aebi Markus, Tanaka Seiji, Muramatsu Sachiko, Araki Hiroyuki, Sonoike Kintake, Nogami Satoru, Morishita Shinichi
Abstract excerpt
One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat...
Topics
- Actins
- Cell Nucleus
- Fungal Proteins
- Gene Deletion
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Techniques
- Genome, Fungal