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Article

High-Throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Mattiazzi Usaj Mojca, Lo Dara S, Grys Ben T, Andrews Brenda J

Abstract excerpt

We describe a protocol for live-cell high-throughput (HTP) screening of yeast mutant strains carrying fluorescent protein markers for subcellular compartments of choice using automated confocal microscopy. This procedure, which combines HTP genetics and microscopy, results in the acquisition of thousands of images that can be analyzed in a systematic and quantitative way to identify morphology defects in the...

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PMID: 34028720
DOI: 10.1007/978-1-0716-1402-0_12
PMCID: PMC9107603

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