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Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer

17 Sept 2021

Abstract excerpt

Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed in a diverse array of high-throughput single-cell "omic platforms," both in an unbiased manner or after selection of...

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PMID: 34585168
DOI: 10.1016/j.xpro.2021.100841

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