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Expression Analysis of Rare Cellular Subsets: Direct RT-PCR on Limited Cell Numbers Obtained by FACS or Soft Agar Assays

1 Apr 2013

Abstract excerpt

Since tissues and tumors are heterogenous populations containing different cell types, their transcriptomes are blends of multiple mRNA expression profiles. Although fluorescence-activated cell sorting (FACS) allows isolation of individual cell types, RNA isolation and quantification remain problematic from rare subsets, such as tissue stem cells. Likewise, identification of transcriptional changes relevant to...

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PMID: 23581467
DOI: 10.2144/000114019

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