Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Protocol for ex vivo culture of patient-derived tumor fragments

6 May 2023

Abstract excerpt

The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryopreservation of PDTFs, and their subsequent thawing. We detail culture of PDTFs and their preparation for analysis. This...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cancer Immunotherapy and Biomarkers
Share to CAR-T cell therapy research
Share to Immunotherapy and Immune Responses

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 37149855
DOI: 10.1016/j.xpro.2023.102282

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Protocol for ex vivo culture of patient-derived tumor fragmentsPMID 37149855Read ↗

A live tumor fragment platform to assess immunotherapy response in core needle biopsies while addressing challenges of tumor heterogeneitySemantic proximity Read ↗

Transcriptomic analysis-guided assessment of precision-cut tumor slices (PCTS) as an ex-vivo tool in cancer researchSemantic proximity Read ↗

Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR-T cell testingSemantic proximity Read ↗

A viable human lung cancer tissue collection (LCTC) to accelerate translational research.Semantic proximity Read ↗

Technologies for deriving primary tumor cells for use in personalized cancer therapySemantic proximity Read ↗

Ex Vivo Tumor Culture Systems for Functional Drug Testing and Therapy Response PredictionSemantic proximity Read ↗

Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancerSemantic proximity Read ↗

Emerging Strategies in 3D Culture Models for Hematological CancersSemantic proximity Read ↗

Protocol for primary human lung organoid-derived air-liquid interface <i>in vitro</i> model to study response to SARS-CoV-2Semantic proximity Read ↗

Rapid Functional Drug Screening of Passage-Zero Patient Brain Tumor Tissues Ex Vivo: Results from a Clinical Feasibility StudySemantic proximity Read ↗

From Patient to Tumor Organoid: Culture Protocol Choice Controls Glioblastoma Tumor Architecture and IdentitySemantic proximity Read ↗

TissueGrinder, a novel technology for rapid generation of patient-derived single cell suspensions from solid tumors by mechanical tissue dissociationSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.