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Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

16 Dec 2021

Abstract excerpt

Skin is our body's outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow...

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PMID: 34977690
DOI: 10.1016/j.xpro.2021.101052

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