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Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing

12 May 2021

Abstract excerpt

Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies. However, human bone marrow stromal cells are difficult to isolate and prone to damage during isolation. This protocol describes a combination of mechanical and enzymatic isolation of BM stromal cells from human BM biopsies,...

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Identifiers

PMID: 34027494
DOI: 10.1016/j.xpro.2021.100538

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