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Factors Affecting Cell Viability during the Enzymatic Dissociation of Human Endocrine Tumor Tissues

27 Aug 2024

Abstract excerpt

The enzymatic dissociation of human solid tissues is a critical process for disaggregating extracellular matrix and the isolation of individual cells for various applications, including the immortalizing primary cells, creating novel cell lines, and performing flow cytometry and its specialized type, FACS, as well as conducting scRNA-seq studies. Tissue dissociation procedures should yield intact, highly viable...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 39336093
DOI: 10.3390/biology13090665

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