Article
Development of multiplex genome editing toolkits for citrus with high efficacy in biallelic and homozygous mutations.
Plant molecular biology - 1 Oct 2020
Huang Xiaoen, Wang Yuanchun, Xu Jin, Wang Nian
Abstract excerpt
KEY MESSAGE: We have developed multiplex genome editing toolkits for citrus that significantly improve citrus genome editing efficacy. CRISPR/Cas systems have been engineered for genome editing in many organisms, including plants. However, the gene editing efficiency in citrus via CRISPR technology remains too low to be implemented for genetic improvement in practice. Moreover, it is very difficult to obtain...
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