Article
Function analysis of RNase E in the filamentous cyanobacterium Anabaena sp. PCC 7120.
Research in microbiology - 1 Jan 2000
Yan Huaduo, Cheng Yarui, Wang Li, Chen Wenli
Abstract excerpt
RNase E is an endoribonuclease and plays a central role in RNA metabolism. Cyanobacteria, as ancient oxygen-producing photosynthetic bacteria, also contain RNase E homologues. Here, we introduced mutations into the S1 subdomain (F53A), the 5'-sensor subdomain (R160A), and the DNase I subdomain (D296A) according to the key activity sites of Escherichia coli RNase E. The results of degradation assays demonstrated...
Topics
- Anabaena
- Bacterial Proteins
- Binding Sites
- Biocatalysis
- Catalytic Domain
- Cell Division
- Cytoskeletal Proteins
- Endoribonucleases
- Models, Molecular
- Molecular Docking Simulation
- Mutation
- RNA, Bacterial
- RNA, Messenger
- Transcription, Genetic
