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Article

CRISPR/Cas9-Mediated Specific Integration of Fat-1 and IGF-1 at the pRosa26 Locus.

Genes - 1 Jul 2021

You Wenni, Li Mengjing, Qi Yilin, Wang Yanbing, Chen Yiwu, Liu Ying, Li Li, Ouyang Hongsheng, Pang Daxin

Abstract excerpt

Many researchers have focused on knock-in pigs for site-specific integration, but little attention has been given to genetically modified pigs with the targeted integration of multiple recombinant genes. To establish a multigene targeted knock-in editing system, we used the internal ribosome entry site (IRES) and self-cleaving 2A peptide technology to construct a plasmid coexpressing the fatty acid desaturase...

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Share to Animals, Genetically Modified
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 34356043
DOI: 10.3390/genes12071027
PMCID: PMC8305104

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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