Article
High-throughput suppressor screen demonstrates that RcsF monitors outer membrane integrity and not Bam complex function.
Proceedings of the National Academy of Sciences of the United States of America - 10 Aug 2021
Tata Muralidhar, Kumar Santosh, Lach Sarah R, Saha Shreya, Hart Elizabeth M, Konovalova Anna
Abstract excerpt
The regulator of capsule synthesis (Rcs) is a complex signaling cascade that monitors gram-negative cell envelope integrity. The outer membrane (OM) lipoprotein RcsF is the sensory component, but how RcsF functions remains elusive. RcsF interacts with the β-barrel assembly machinery (Bam) complex, which assembles RcsF in complex with OM proteins (OMPs), resulting in RcsF's partial cell surface exposure....
Topics
- Bacterial Capsules
- Bacterial Outer Membrane Proteins
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Suppressor
- High-Throughput Screening Assays
- Lipopolysaccharides
- Multiprotein Complexes
- Mutation
- Phenylpropionates