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Article

Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors.

BMC genomics - 22 Jul 2021

Straume Anne Hege, Kjærner-Semb Erik, Skaftnesmo Kai Ove, Güralp Hilal, Lillico Simon, Wargelius Anna, Edvardsen Rolf Brudvik

Abstract excerpt

BACKGROUND: New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes breeding an unattractive solution to obtain homozygous mutants and improving the rates of perfect HDR in founder (F0) fish is thus required. Genome editing can represent small DNA changes down to...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 34294050
DOI: 10.1186/s12864-021-07823-8
PMCID: PMC8296724

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