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Evaluating coverage bias in next-generation sequencing of Escherichia coli

24 Jun 2021

Abstract excerpt

Whole-genome sequencing is essential to many facets of infectious disease research. However, technical limitations such as bias in coverage and tagmentation, and difficulties characterising genomic regions with extreme GC content have created significant obstacles in its use. Illumina has claimed that the recently released DNA Prep library preparation kit, formerly known as Nextera Flex, overcomes some of these...

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PMID: 34166413
DOI: 10.1371/journal.pone.0253440

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