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Article

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

Scientific reports - 4 Sept 2020

Nouws Stéphanie, Bogaerts Bert, Verhaegen Bavo, Denayer Sarah, Piérard Denis, Marchal Kathleen, Roosens Nancy H C, Vanneste Kevin, De Keersmaecker Sigrid C J

Abstract excerpt

Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate characterization and relatedness determination. However, standardized and harmonized workflows, e.g. for DNA extraction, are required to ensure robust and exchangeable WGS data. Data sharing between (inter)national laboratories is essential to support foodborne pathogen control, including outbreak investigation. This study...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32887913
DOI: 10.1038/s41598-020-71207-3
PMCID: PMC7474065

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