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Cell culture systems for isolation of SARS-CoV-2 clinical isolates and generation of recombinant virus

10 Apr 2023

Abstract excerpt

A simple and robust cell culture system is essential for generating authentic SARS-CoV-2 stocks for evaluation of viral pathogenicity, screening of antiviral compounds, and preparation of inactivated vaccines. Evidence suggests that Vero E6, a cell line commonly used in the field to grow SARS-CoV-2, does not support efficient propagation of new viral variants and triggers rapid cell culture adaptation of the...

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PMID: 37095858
DOI: 10.1016/j.isci.2023.106634

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